hybridization$36360$ - significado y definición. Qué es hybridization$36360$
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Qué (quién) es hybridization$36360$ - definición

METHOD FOR DETERMINING THE CONSTITUENT NUCLEOTIDES OF A FIXED SIZE IN A STRAND OF DNA
Sequencing by Hybridization

Nucleic acid hybridization         
PHENOMENON IN MOLECULAR BIOLOGY
Hybridisation (molecular biology); DNA hybridization; Dna hybridization; Nucleic acid hybridisation; Hybridization in molecular biology; Hybridization (molecular biology)
In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules anneal to complementary DNA or RNA. Though a double-stranded DNA sequence is generally stable under physiological conditions, changing these conditions in the laboratory (generally by raising the surrounding temperature) will cause the molecules to separate into single strands.
Comparative genomic hybridization         
  • ACGH profile of the IMR32 neuroblastoma cell line
  • Figure 2. Array-CGH protocol
METHOD TO EVALUATE BIOLOGICAL SAMPLES
Comparative genomic hybridization (CGH); Array-comparative genomic hybridization; ACGH; Array CGH; Comparative Genomic Hybridization; Comparative Genomic Hybridisation; Comparative hybridization; Array comparative genomic hybridisation; Cot 1 DNA; Cot-1 DNA; Comparative genome hybridization; Chromosomal microarray analysis; CGH array; Array comparative genomic hybridization; Array-comparative Genomic Hybridization; Microarray-based comparative genomic hybridization; A-CGH; Array hybridization
Comparative genomic hybridization (CGH) is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome).
fish test         
  • A metaphase cell positive for the ''bcr/abl'' rearrangement (associated with [[chronic myelogenous leukemia]]) using FISH. The chromosomes can be seen in blue. The chromosome that is labeled with green ''and'' red spots (upper left) is the one where the rearrangement is present.
  • Scheme of the principle of the FISH Experiment to localize a gene in the nucleus.
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  •  doi = 10.1186/s13039-022-00622-0 }}</ref>
  • ViewRNA detection of miR-133(green) and myogenin mRNA (red) in C2C12 differentiating cells
  • Urothelial cells marked with four different probes
GENETIC TESTING TECHNIQUE
Chromosome painting; In situ hybridization, fluorescence; Fluorescence In Situ Hybridization; Fluorescence In-Situ Hybridisation; Fluorescence In-Situ Hybridization; Fluorescent in situ hybridisation; Fluorescent in-situ hybridization; FISH (genetic); Fish assay; Fluorescent in situ hybridization; Fluorescence in situ hybridisation; FISH test; SmFISH
How to tell genuine surrealistic invention from cod surrealism - that perpetrated by people who think that surrealism involves a fish up a tree, a man holding a fish outside a toilet, a fish and a lettuce hanging from the ceiling, men with fish in their mouths. See reprehensible UK comedy Dare To Believe for the worst example of this.
Uh-oh, here's a new off-the-wall comedy. Time to apply the fish test.

Wikipedia

Sequencing by hybridization

Sequencing by hybridization is a class of methods for determining the order in which nucleotides occur on a strand of DNA. Typically used for looking for small changes relative to a known DNA sequence. The binding of one strand of DNA to its complementary strand in the DNA double-helix (known as hybridization) is sensitive to even single-base mismatches when the hybrid region is short or if specialized mismatch detection proteins are present. This is exploited in a variety of ways, most notably via DNA chips or microarrays with thousands to billions of synthetic oligonucleotides found in a genome of interest plus many known variations or even all possible single-base variations.

The type of sequencing by hybridization described above has largely been displaced by other methods, including sequencing by synthesis, and sequencing by ligation (as well as pore-based methods). However hybridization of oligonucleotides is still used in some sequencing schemes, including hybridization-assisted pore-based sequencing, and reversible hybridization.